Dye for Mindray 5-Qeyb ka mid ah Falanqeeyayaasha Hematology Hematology waxaa ku jira polymethine gaar ah iyo midabyo fluorescent nucleic acid ah kuwaas oo si gaar ah ugu xidhan DNA-da gacanta iyo RNA. Reagen-yaashani waxay calaamadiyaan unugyada dhiiga cad, reticulocytes, iyo platelets gudaha unugga socodka, taas oo awood u siinaysa kala soocida kala firdhisanaanta cabbirka badan iyadoo loo marayo kala firdhisanaanta iftiinka laser semiconductor iyo ogaanshaha fluorescence.

Waa maxay Hematology Stain/Dye Reagents?

Dib-u-hagaajinta dhiig-baxa wasakhda/dheeha ee loogu talagalay falanqeeyayaasha otomaatiga ah waa xal kiimiko oo ka kooban fluorophores organic, surfactants, iyo stabilizers osmotic. Si ka duwan saxarada dhiiga smear-ka ee gacanta, reage-yaashan dareeraha ah ee dareeraha ah waxay ku shaqeeyaan gudaha -nidaamka cytometry socodka dareeraha otomaatiga ah.

Marka lagu duro qolka falcelinta ogaanshaha, dheehu wuxuu dalaa xuubka unugyada ee ay diyaariyeen reagents lyse gaar ah wuxuuna ku xidhaa stoichiometrically iyo asiidhyada unugyada nucleic-ka. Marka lagu soo bandhigo laydhka laysarka ee semiconductor gudaha unugga socodka, fluorophores-yada xidhxidhan waxay sii daayaan iftiinka dambe ee fluorescent oo u dhiganta nuxurka aashitada gacanta.

Doorka Kala Soocida / Ogaanshaha Unugyada

Dib-u-hawlgalayaasha dheeha fluorescent ayaa awood u siinaya kala soocida indhaha ee unugyada dhiigga ee adag:
Calaamadaynta Asiidhka Nukliyeerka:Fluorophores waxay isu beddelaan RNA intracellular iyo strands DNA. Unugyada leh dhaqdhaqaaqa dheef-shiid kiimikaad sarreeya ama marxalado aan qaan-gaarin waxay nuugaan uruurinta dheeha sare, iyagoo soo saaraya calaamado dhaadheer oo xooggan.
Jiilka Scattergram:Isku darka iftiinka kala firdhiso dhinaca iyo xoojinta iftiinka dambeedka waxay abuurtaa{0} kala firdhiso cabbirro badan.
Baaritaanka Unugyada aan Caadiga ahayn:Noocyada qiiqa sii daaya ee kala duwan ayaa ka soocaya lymphocytes-ka caadiga ah, granulocytes aan qaan-gaarin, iyo unugyada dhiigga cas ee nukleed ee unugyada dhiiga cad ee qaangaarka ah.

Kanaalada Ogaanshaha

Kanaalka Ogaanshaha	Magaca Kanaalka / Bartilmaameedka	Hawlaha Bayoolojiga & Habka Cabirka
Kanaalka WNR	Unug cad oo Nukliyeer ah & Kanaalka RBC	Wuxuu cabbiraa wadarta tirada WBC wuxuuna tiriyaa NRBC. Basophils waxaa lagu kala soocaa iyada oo loo sii marayo kala firdhisanaan jireed halka NRBC asiidhyada nucleic-ka lagu calaamadeeyay si fluorescently.
Kanaalka WDF	Kanaalka Kala Duwan ee Unug Cad	Kala soocida Neutrophils, Lymphocytes, Monocytes, iyo Eosinophils. Wuxuu cabbiraa Granulocytes aan qaan-gaarin iyadoo loo marayo nucleic acid fluorescent dheeha xoogga xira.
Kanaalka WPC	Kanaalka Hordhaca Unug Cad	Baaritaanada unugyada dhalooyinka, lymphocytes aan caadi ahayn, iyo qaraxyada. Wuxuu adeegsadaa falcelinta xuubabka gaarka ah oo ay weheliso midabaynta si loo kala saaro unugyada qaraxa.
RET Channel	Kanaalka Reticulocyte	Wuxuu cabbiraa saamiga Reticulocyte, tirinta saxda ah, Jajabka Reticulocyte ee aan qaan-gaarin, iyo unugyada dhiigga cas ee u dhigma cabbirrada iyada oo loo marayo midabaynta fluorescent RNA.
Kanaalka PLT	Platelet Optical Channel	Waxay bixisaa tirinta platelet indhaha iyadoo la isticmaalayo calaamadaynta dheeha fluorescent ee platelet RNA, ka hortagga faragelinta RBC-yada microcytic ama burburka.

Taxanaha Falanqeeyaha Waafaqsan

Taxane 5-Qayb Laser Hematology:Loo qaabeeyey nidaamyada dareeraha ah ee loo isticmaalo semiconductor laser fluorescence socodka cytometry.

Sare -Xarumaha Shaqada Dhiiga Dhiiga ee Madhxiyaha ah:La jaanqaadi kara daboolka tooska ah{0}}Sambalayaasha daloolista iyo xadhkaha dareeraha ah ee rarida joogtada ah.

Isku-dhafka Dhiigbaxa -Nidaamka Sameeyaha Islaaydh:Loo ansixiyay nidaamyada gudbinta tooska ah ee khadka tooska ah iyada oo aan lahayn calaamad u leexasho ama kala duwanaansho cadaadis.

Tilmaamaha Alaabta

Halbeegga	Heerka Farsamada / Xogta
Nooca habaynta	Xalka dheeha fluorescent dareere ah oo urursan
Saldhig Fluorophore Firfircoon	Polymethine / Nucleic acid dheeha fluorescent
Dhererka mowjadda xiisaha leh	633 nm - 635 nm
Heerka pH (25 darajo)	7.00 – 8.50
Waxqabadka (25 darajo)	1.20 - 2.50 mS/cm
Cufnaanta (20 darajo)	1.005 - 1.020 g/cm³
Furan -Xasilloonida Vial	60 maalmood (2 darajo - 30 darajo kaydinta mugdiga ah)
Nolosha shelf ee aan furnayn	18 bilood
Heerkulka Kaydinta	2 darajo - 30 darajo (Ka ilaali iftiinka iyo barafowga)
Shaandheyn gaar ah	Lagu sifeeyay 0.2 µm xuub

Codsiga & Shaqada Reagent

Falanqaynta Kala Duwan ee WNR:Lagu duray qolka falcelinta WNR si loo calaamadiyo unugyada dhiigga cas ee nukleed iyo nukleiyada unugyada dhiiga cad ka dib kala dirida cytoplasm.

WDF 5-Qayb ka soocida WBC:Waxay la falgalaan dad-hoosaadyada WBC ee kanaalka WDF, iyagoo soo saaraya kooxo fluorescence kala duwan oo loogu talagalay lymphocytes, monocytes, neutrophils, iyo eosinophils.

Hordhac & Calanka Unugga Qaraxa:Loo qaabeeyey in si door ah loo galo xuubabka dufanka ee la beddelay ee kanaalka WPC, taas oo u oggolaanaysa aqoonsiga dadyowga unugyada aan qaan-gaarin.

Tirinta Reticulocytes RNA:Waxay ku xidhan tahay cytoplasmic ribosomal RNA ee reticulocytes, taas oo u oggolaanaysa xisaabinta marxaladaha korriinka reticulocyte.

Tirinta Platelet indhaha:Waxay wasakhaysaa jajabyada RNA gudaha ee platelets dhiiga si ay u dhaliyaan tirooyinka indhaha ee hoos yimaada - xaaladaha caafimaad ee platelet

Xidhmada / Kaydinta

Shayga	Tilmaamid
Baakadaha caadiga ah	12 mL x 1 dhalo / sanduuq, 42 mL x 1 dhalo / sanduuq
Walxaha dhalada	Iftiin - dhalooyin HDPE leh oo cambar ah oo gaashaan leh
Heerkulka Kaydinta	2 darajo ilaa 30 darajo
Shuruudaha Gaadiidka	Ka ilaali qaboojinta iyo iftiinka qorraxda tooska ah

Reagents Hematology La Xiriira

Xalka Dheefta leh ee 5-Qayb ee Falanqeeyayaasha Dhiigga:Xalka isotonic conductive baffer ee loogu talagalay milanka saamiga dhiigga iyo xakamaynta dhawaaqa asalka ah.

Lyse Reagent ee 5-Qayb ee Hematology Analyers:Surfactant{0}}Xalka beenta ku salaysan ee kala dirida xuubabka erythrocyte.

Xalka Nadiifinta Xoogan:Nadiifiyaha dareeraha ah ee dayactirka khadka dareeraha tooska ah iyo ka saarida dhigaalka borotiinka.

Nadiifiyaha Baarista:Walxaha dhaqida shaybaarka ee alkaline hypochlorite ee muunadda barootiinaynta.

FAQ

S: Sidee buu u toosan yahay reagent-ka fluorescent-ka kaga duwan yahay reageniyeyaasha wasakhaynta dhiigga ee dhaqameed?

J: Wasaqda smear-dhaqameedka waxay u baahan tahay in gacanta lagu qalajiyo sawirada muraayadaha si loogu qiimeeyo muuqaal ahaan iyada oo hoos timaada mikroskoob. Qalabaynta dheeha oo otomaatig ah ayaa ah dheeha dheeha-biyoodka ah ee loo qaabeeyey qolalka falcelinta khadka. Waxay ku xidhaan RNA/DNA-ga gacanta ee hakin dareere ah, iyagoo soo saaraya calaamadaha iftiinka ee lagu ogaado cytometry socodka laysarka.

S: Soo-gaadhista iftiinka miyuu saameeyaa waxqabadka dheeha inta lagu jiro hawlgallada maalinlaha ah?

J: Haa. Fluorophores-yada firfircoon waxay hoos u dhigaan soo-gaadhista iftiinka tooska ah ama iftiinka UV-ga ee muddada dheer. Reagen-yada waxa lagu baakadeeyay iftiin - weelasha cambarka gaashaman waana in ay ku jiraan qolka reageent ee xidhan inta lagu jiro hawlgalka

S: Waa maxay heerkulka kaydinta loo baahan yahay inta lagu jiro gaadiidka iyo bakhaarka?

A: Ku kaydi reajentiyeyaasha inta u dhaxaysa 2 digrii iyo 30 digrii. Kaydinta ka hooseeya 0 digrii waxay sababtaa roobab fluorophore. Heerkulka ka sarreeya 35 digrii wuxuu dedejiyaa hoos u dhaca walxaha firfircoon.

S: Sidee buu wax badan u saameeyaa natiijooyinka kala firdhiso garaafka?

J: Kala duwanaanshaha u-fiirsashada dheeha ayaa sababa isbeddelada kutlada kala firdhisan, taasoo horseedaysa khaladaadka gating. Wax soo saar kastaa wuxuu maraa xaqiijinta nadiifnimada fluorophore, spectrofluorometric test, iyo matrix u dhigma si loo joogteeyo meelaynta kala firdhisanaanta joogtada ah.

S: Waa maxay naqshadaynta baakadaha loo isticmaalo isku xirka dareeraha?

J: Reagennada dheeha waxaa lagu bixiyaa dhalooyin HDPE leh oo daboolan oo wata koofiyadaha loogu talagalay khadadka qaadashada dareeraha tooska ah, ka hortagga uumiga iyo wasakheynta.

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